Journal: eLife
Article Title: Cytoneme-mediated intercellular signaling in keratinocytes is essential for epidermal remodeling in zebrafish
doi: 10.7554/eLife.97400
Figure Lengend Snippet: RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.
Article Snippet: Commercial assay or kit , SuperScript III CellsDirect cDNA Synthesis Kit , Fisher Scientific , 18-080-200 , .
Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Positive Control